The Effect of combination therapy of Caffeine and Glibenclamide on Oxidative Stress in the liver Tissue among Streptozotocin-Induced Diabetic Rats

Background and Objective:The Oxidative stress caused by diabetes, shows an imbalance between free radicals and antioxidant defense system; which has a destructive effect on the liver structure and function. Glibenclamide is one of the materials which is widely used in diabetes, but has a lot of side effects such as hypoglycemia and loss of consciousness; nowadays Caffeine used in various countries and has anti-oxidant effect; but the effect of caffeine on the therapeutic role of Glibenclamide is undetermined. This study was conducted to survey the combination therapy effect of caffeine and Glibenclamide on the catalase activity as an oxidative stress index in the liver tissue among Streptozotocin-Induced Diabetic Rats.Materials and Methods: In this experimental investigation, 32 male rats, weighing 200 - 250 g, were divided into four groups: 1: diabetic without treatment, 2: diabetic receiving treatment with Glibenclamide, 3: diabetic receiving treatment with caffeine, and 4: diabetic receiving treatment with glibenclamide and caffeine. Diabetes was induced by intraperitoneal injection of Streptozotocin (60 mg/kg). On the third day after the injection to ensure the mice are diabetic, by using glucometer we measured the blood sugar and A value of blood sugar above 250 mg/dl was chosen to be the reference for deciding whether they had diabetes or not; diabetic rats chose for the next step; Treated group with glibenclamide get daily 0.3cc, treated group with caffeine get daily 0.5cc and the group treated with glibenclamide and caffeine get daily respectively, 0.15cc and 0.25cc by intraperitoneal injection. In the second week after drug administration, rats were anesthetized and by opening the peritoneal cavity removed the liver tissue and after homogenizing and centrifuging, by using supernatant, the Catalase activity of the liver was measured by Beer & sizer method. Quantitative data were analyzed using one-way ANOVA. Results: First we knew that all the rats are diabetic because their blood sugar is above 250 mg/dl. Treatment of diabetic group with caffeine non-significantly reduced catalase activity in liver tissue versus untreated diabetic group (p> 0.05). In addition, glibenclamide treatment of diabetic group also non-significantly increased catalase activity versus untreated diabetic group (p> 0.05). Meanwhile, a similar condition occurred for combination therapy with glibenclamide and caffeine of diabetic group in comparison with diabetic group.Conclusion: It was found out that caffeine and glibenclamide alone or in combination could not significantly improve catalase activity as a defensive system in liver tissue.

کلیدواژه ها:

Diabetes Mellitus ، Oxidative Stress ، Liver ، Rat ، Caffeine ، Glibenclamide