Effect of culture-medium type and temperature on pollen germination of medicinal plant Leonurus cardiaca L.

Leonurus cardiaca L., commonly known as motherwort, is a member of the Lamiaceae family. It has been used to cure cardiovascular problems, stress and nervous irritability. In vitro pollen germination is a very convenient and effective technique to study many basic and applied aspects of pollen biology. Review in the literature revealed that there is no report on the pollen biology of this species. Therefore, this experiment was designed to investigate influence of culture media with different concentrations of boric acid (75, 100 and 125 mg/l) and sucrose (10 and 15 %) in liquid and solid cultures on pollen germination and effect of temperature (10, 25 and 35 ℃) on in vitro pollen germination of L. cardiaca. Results show that the pollen germination is highest in solid medium and a combination of 15 % sucrose, 100 mg/l boric acid and 1 % agar (82.96 %). Maximum pollen germination occurred at temperature of 25˚C.

کلیدواژه ها:

Motherworth ، pollen germination ، temperature