Fabrication of a Glucose Nanobiosensor based on Silver Nanoparticles and Glucose Oxidase-Chitosan Matrix

A nanobiosensor based on silver nanoparticles (AgNPs) was fabricated for glucose detection in aqueous phase. Glucose oxidase was mixed with chitosan and dropped on a platinum (Pt) electrode. Modified electrode was coated with AgNPs using electrodeposition method. The electrochemical performance of the modified electrode was evaluated by cyclic voltammetry and amperometry.The fabricated biosensor had a high sensitivity of 58.6 μA mM−1 cm−2 and detection limit of 4.4 μM glucose at a signal to noise ratio of 3. In addition, the biosensor showed a short response time less than 5 s and a wide linear range of 0.05-11.5 mM. The apparent Michaelis–Menten constant (KM) was found to be 9.14 mM. In addition, thermal stability and anti-interference ability of the biosensor were investigated. The results demonstrated that AgNPs enhanced the analytical performance of the biosensor.

کلیدواژه ها:

Nanoiosensor ، Silver nanoparticles ، Amperometry ، Chitosan ، Glucose oxidase