Evaluation of the effect of autophagy induction and inhibition on Street rabies virus titer in NMRI murine model by real-time PCR

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 451

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شناسه ملی سند علمی:

CIGS15_501

تاریخ نمایه سازی: 13 بهمن 1398

چکیده مقاله:

Background and Aim : Rabies is the most common zoonotic disease in the country. Autophagy is a vital process that maintains homeostasis by removing the harmful components of cytoplasm and can be used as an inherent defense mechanism against viruses. Increasing knowledge about the interaction between rabies virus and autophagy pathway genes and effect of host cell autophagy on rabies virus replication may lead to new antiviral treatments.Materials and Methods: Six groups of NMRI mice were treated by 3-methyl adenine, rapamycin, sterile phosphate buffer, street rabies virus, rapamycin plus rabies virus and 3-methyl adenine plus rabies virus. Drugs and virus were injected intra cranially . Street rabies virus titer and Map1lc3 gene expression were detected by Real-time PCR and cell death in apoptosis was measured by TUNEL Assay and LC3B protein level was measured by Immunohistochemistry. Results: Mapl1c3 gene expression in viral specimens increased over all hours compared with normal specimens. The level of LC3B proteins were also increased during street rabies virus infection, but the number of apoptotic cells did not increase. Doses of rapamycin and trimethyl adenine drugs used in this study did not have any effect on the rabies virus titer Conclusions: The street rabies virus induces autophagy in brain of infected mice, which could be result of the innate immune system of the cells inhibition of virus activity but autophagy induction and inhibition did not have any effect on Street rabies virus titer in brain of infected mice. The street rabies virus has no apoptotic effect

نویسندگان

Seyedeh Fatemeh Hosseini Heydarabadi

Department of Genetics, Faculty of Basic science, Science and Research branch, Islamic Azad University, Tehran, Iran.

Farzaneh Sheikholeslami

Rabies laboratory, Virology Department, Pasteur Institute of Iran

Iman Salahshourifar

Department of Genetics, Faculty of Basic science, Science and Research branch, Islamic Azad University, Tehran, Iran.