Study of Trypsin Behavior into Functionalized Nano-Engineered SBA-15: Loading and Stability of Enzyme

Immobilization of trypsin enzyme (37×37×42A°) into inorganic host materials has attracted considerable attention over the past few years. Hexagonal mesoporous SBA-15-type molecular sieves with thiol, amin, phenyl, sulfonic acid functional groups and pore size in the range of 55-65A° have been prepared non-ionic block copolymers and used for immobilization of the trypsin enzyme. Trypsin immobilization on unfunctionalized SBA-15 showed the maximum amount of loading and activity at 25 °C, PH 8 and lowest amount of medium ionic strength. Though about the functionalized SBA-15, we see that each function has a specific effect on trypsin behavior. The XRD and pore symmetry result indicated that SBA-15 with thiol function has a maximum amount of loading due to our experimental data. However, in this report in temperature over than 40°C, could not be achieved a good activity from immobilized trypsin although stability of adsorbed trypsin in comparison to free one was totally considerable. Our focus in this study is on SBA-15 with thiol and sulfonic acid functions, because of the maximum activity and highly amount of loading could be accomplished in this condition.

کلیدواژه ها:

Mesoporous Materials ، Immobilization ، Enzyme Assay ، Trypsin