Antibacterial activity of methanol extract from Cotoneaster nummularioides, Cynodon dactylon and Cardaria draba on typical food-born pathogens.

The present study aimed at evaluating the in vitro antibacterial activity of methanol extract of Cotoneaster nummularioides, Cynodon dactylon and Cardaria draba against different pathogenic microorganisms. The agar disk diffusion method was used to study the antibacterial activity of Cotoneaster nummularioides, Cynodon dactylon and Cardaria draba methanolic extracts against 2 gram-positive and 2 gram-negative bacteria at 300 and 400 mg/ml. The results revealed that the methanol extract of Cotoneaster nummularioides presented the highest zone of inhibition against tested pathogens (7-12 mm inhibition zones). Other plants did not showed significant zone inhibition. Minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were quantified by micro-dilution method. The leaf extract of Cotoneaster nummularioides against Bacillus cereus (PTTC 1015) and Staphylococcus aureus (PTTC 1431) strains showed the best activities, with the lowest minimal inhibitory concentration (MIC) of 3.125 mg/ml and MBC was 102.08 mg/ml for Staphylococcus aureus (PTTC 1431) and 108.33 mg/ml for Bacillus cereus.Results here show that the methanol extract of this plants antibacterial activity and therefore it could be used as a natural preservative ingredient in food and/or pharmaceutical industries.

کلیدواژه ها:

Cotoneaster nummularioides ، Cynodon dactylon ، Cardaria draba ، methanol extract ، Antibacterial activity