Identification and Comparison of the Chloroplast DNA trnL-F of the Populus caspica Populations from the Hyrcanian Forests; North West of Iran

The genus Populus is important member of Salicaceae.In the world have been identified more than 20 speciesof Salix and 50 species of populus. In addition havebeen described hundreds of varieties and hybrids.Since the trees are amplified in natural methods (sexualreproduction) and also in shoot cuttings method,because of hybridization between them, identificationis very difficult to identify. Populus caspica is native inHyrcanian forests and they usually grow in light-moistureis regions. The purpose of this study is indentification ofchloroplast genome based on the nucleotide sequenceof the non-coding regions cpDNA (trnL-F) in the P.caspica populations and comparison with other Populusin the NCBI gene bank. Two samples from seven sitesof P. caspica, were collected. Total genomic DNA wasextracted from frozen leaf tissues using of the Doyleand Doyle method. Polymerase chain reaction (PCR)performed by using of Pfu DNA polymerase andoligonucleotide primers UEUFF/UEUFR which designedby Taberlet et al. (1991). The chromatograms of the DNAsequencing results were processed and analyzed by theBlast software and were sequenced with other sequencesof pupulus finding in gene bank with ClustalW ver.2.Results showed that P. caspica in all populations withthe same sequence of 339 nucleotide is different with P.nigra (281 nucleotide), P. tremuloides (224 nucleotide),P. nigra (228 nucleotide) that are in gene bank. As inall populations samples P.caspica were observed 112nucleotides substitutions, 78 insertions and 27 gapsthat were differed from other sequences. Based ourfunding, recommend that trnL-F marker can be used asa molecular marker for identification propos.

کلیدواژه ها:

Populus caspica ، Populus nigra ، Choloroplast DNA ، trnL-F ، PCR