Hepatic ischemia-reperfusion injury in different species: Comparison between a lower species (sting ray fish) and a higher species (rat)

Objective: The mechanisms by which the injury induced by ischemia-reperfusion in the liver are under intensive investigations. However, the controversy and unclear problems remain to be known. One of these unknown issues is the features of the injury and the level of hepatic resistance against reperfusion injury in aquatic species including sting ray. The present study aimed to characterise the functional and structural changes in the stingray livers exposed to ischaemia-reperfusion in comparison with rat. Method & Materials: Using an isolated perfused model, the sting ray and rat livers were subjected to 60 min ischemia followed by 60 min reperfusion. Functional and morphological changes were examined by oxygen consumption, bile production, enzyme release, lignocaine extraction, and histological observations. Results & Conclusion: It was found that the effects of reperfusion injury on the function of the liver of sting ray and rat were relatively the same. The occurrence of apoptosis was the most important histological alterations in the livers of both species. However, it was appeared that the liver of the sting ray was more resistant to that ischaemia-reperfusion injury than the rat liver. It can be concluded that the liver of low species is more resistant to the ischemia-reperfusion. It is suggesting that the low O2 demand and lack of efficient ROS production leading to reperfusion injury, may be the cause for the resistant

کلیدواژه ها:

Isolated liver ، Rat ، Sting ray ، Reperfusion injury ، Apoptosis