Novel single and hybrid immobilization methods for enhanced phenol degradation by Pseudomonas sp. SA01
سال انتشار: 1388
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 1,457
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شناسه ملی سند علمی:
ICAWTR01_154
تاریخ نمایه سازی: 20 اردیبهشت 1390
چکیده مقاله:
In this investigation, single and hybrid immobilization techniques for whole cells of Pseudomonas sp. SA01 were employed to enhance phenol degradation rate. The single immobilization was achieved using alginate (2-4%) and pectin (3-5%) together with the combined immobilization systems which consisted of alginate-chitosan-alginate (ACA), polyvinyl alcohol (PVA)-alginate capsules. The freely suspended cellsof Pseudomonas sp. SA01 was able to degrade phenol in concentrations up to 1000 mg/L 84 h. The complete degradation of the same concentration of phenol by cells entrapped in alginate (3%), in hybrid immobilization system alginate-chitosan-alginate (ACA), and in PVA-alginate encapsulated cells were achieved as low as 30 h. However, the cells immobilized in pectin (5 %) could degrade the same concentration of phenol at 35 and 40 h respectively. The cell loading capacity is increased in PVA-alginate hybrid capsules owing to the reduction of cell leakage from the beads, resulted in higher activity of immobilized cells compared with the single immobilization method.
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نویسندگان
S Abdollahpour
National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O.BOX ۱۴۱۵۵-۶۳۴۳, Tehran, Iran - Department of Clinical Biochemistry, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran
M Mollaei
National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O.BOX ۱۴۱۵۵-۶۳۴۳, Tehran, Iran
S Atashgahi
National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O.BOX ۱۴۱۵۵-۶۳۴۳, Tehran, Iran
A Sahebghadam Lotfi
Department of Clinical Biochemistry, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran