Direct and indirect plant regeneration in Hypericum perforatum: an important medicinal plant

سال انتشار: 1395
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 594

فایل این مقاله در 15 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

ICESCON04_183

تاریخ نمایه سازی: 25 آذر 1395

چکیده مقاله:

Hypericum perforatum L. (St. Johns’ wort) is the most commercially important species of the genus Hypericum and contains a wide range of components including naphthodianthrones, phloroglucinols, tannins, xanthones, phenolic acids and essential oil. A method for rapid micropropagation of Hypericum through plant regeneration from leaf and stem explant derived calli has been developed. The stem and leaf segments were cultured on MS medium supplemented with the combination of auxins and cytokinin for callus induction. Also, the best callaus induction treatment was selected for evaluation the effect of light and dark conditionon callus quality. By using sterile seedlings, effects of type and concentration of cytokinin and auxin were evaluated on the shoot, callus and root induction. The results showed that the medium containing low BA concentration (0.1mg/l BA) with 0.05 mg/l IBA is the best medium for producing maximum shoot number as well as normal shoots. However, high concentrations of BA (0.1 to 0.25 mg/l) were produced abnormal shoots. This plant also had high ability of shoot induction. These shoots were rooted easily even in culture medium free growth regulators.0.5 mg/l IBA was the best IBA concentration for root induction from produced shoots. Callus obtained from leaf explants had more growth in darkness. The highest callus fresh weight was observed on 0.25mg/l 2,4-D and 1 mg/l kin and also 0.5 mg/l 2,4-D and 1 mg/l BA. The plantlets were acclimatized and transferred to the greenhouse with 80% survival. This in vitro propagation protocol should be useful for conservation as well as mass propagation of this medicinal plant.

نویسندگان

Manizhe Abdollahpoor

Department of Horticultural Science, Tehran Universitty , Karaj , Iran

Siamak Kalantari

Assistant prof, Department of Horticultural Science, Tehran Universitty, Karaj, Iran

Majid Azizi

Professor, Department of Horticultural Science, Ferdowsi University of Mashhad, Mashhad, Iran

Yusef Ali Saadat

Research Assistant prof, Central Agriculture and Natural Resource of Fars Province, Shiraz, Iran

مراجع و منابع این مقاله:

لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :
  • I1] _ .Elgam mal, C. S.I.e.(2009). Tracking People _ Irus, ...
  • L. Zhang, Y. Ii R Nevatia (2(8) _ IData Associatin ...
  • I X.X.hao, _ (Generative Tracking of 3) Huma Motion by ...
  • M.W.Lcc .R.Nevatia .(2009). _ Posc Tracking in Monoclar Sequcncc Using ...
  • _ _ , C. .Iaylor.(2004). Statistical Models of Appearance for ...
  • _ _ _ _ ling [8I A. Yilmaz, _ .Shah.(2006). ...
  • J.1lan, Z. Pan.(2008). Rolbust Moving (Obiccts]Dctcctin in Dymamics Sccnucs Based ...
  • _ Mikami, _ Otsuka, J. Yamato).(2009). Memory-bascd partical filter for ...
  • نمایش کامل مراجع