Highly efficient purification of 6×histidine-tagged recombinant protein-A by MnFe2O4@NH2@2AB-Ni as a novel and highly stable magnetic nanoparticles

In this study we have developed a simple, cheap, and rapid method for purification of 6×histidine-taggedrecombinant proteins with high yields. The new immobilized metal ion affinity adsorbent was synthesized followingco-precipitation method in order to synthesis of superparamagnetic manganes ferrit nanoparticles, and subsequentlycoated with 3-aminopropyltriethoxysilane (APTES) through a silanization reaction. Manganese ferrite nanoparticlesafforded bidentate ligands as a result of reaction between isatoic anhydride and amino-functionalized MnFe2O4. Thestable surface functionalized nanoparticles were further linked with Ni2+ for purification of 6× His-tagged proteins. Thenanoparticles were approximately 75 nm in size and were stable and had negligible non-specific binding for protein.

کلیدواژه ها:

Magnetic nanoparticles ، Immobilized metal ion affinity ، 6×His-tag protein ، Proteins purification