Novel immunotherapeutic trimethyl Chitosan-recombinant protein Hp-Nap of helicobacter pylori Nanocomplex as Breast Cancer treatment

Breast cancer is one of the leading causes of death in women around the world with a very highdegree of mortality and morbidity. The Helicobacter pylori neutrophil-activating protein (HP-NAP)promotes their endothelial adhesion and the production of oxygen radicals and chemokines. HP-NAP is animmune modulator able to induce the expression of IL-12 and IL-23. Chitosan is biodegradable andbiocompatible component. It is low toxicity effect, so apply in drug delivery targets. The aim of studyisNovel immunotherapeutic Chitosan-recombinant protein Hp-Nap of helicobacter pylori Nanocomplex asBreast Cancer treatment. Chitosan Nanoparticles were produce. The size and morphology of thenanoparticles were investigated. recombinant HP-NAP Helicobacter pylori were produce.SDS-PAGEanalysis showed the expression of an approximately 20000 Dalton protein. DLS confirm size and zetapotential of the nanoparticle.Tunnel and flow cytometry confirm apoptosis.The complex has the potential toshift antigen-specific T-cell responses from a predominant Th2 to a polarized Th1 cytotoxic phenotype,characterized by high levels of interferon-γ and tumor necrosis factor-a production. HP-NAP may be a newtool for future therapeutic strategies aimed in cancer immunotherapy. Nanomaterials have been used toenable drug delivery with lower toxicity to healthy cells and enhanced drug delivery to tumor cells.Keywords: Breast cancer, Recombinant HP-NAP, Chitosan.

کلیدواژه ها:

Breast cancer ، Recombinant HP-NAP ، Chitosan