Stress response to transportation on hematological profile of Common Carp (Cyprinus carpio L.)

In this study the stress responses and changes hematological factors were investigated in Carp (Cyprinus carpio L.) juveniles during of Twelve-hour transportation and different salinity and temperature of water of plastic bags. For this purpose, common carp juveniles were exposed to concentration of 0.5, 5 g L-1 salinities and water temperature of 10oC, 18oC and 25oC. Fish (weight 50.50±10.20 g, n=300) were caught from the earth pond and held in one indoor 2000-L fiberglass tank for resting and adaptation at 10 days in laboratory condition. Fish were placed in 40-L polyethylene bags with 20L of water at a density of 40 fish per bag (200g/L). Commercial table salt (NaCl, 97%) was added to the water at 0.5, 5 g L-1 (3 replicates for each concentration) and fresh water with temperature of 10oC, 18oC and 25oC (3 replicates for each temperature). At the beginning and the ending of transporting period the values of erythrocyte count (RBC), hemoglobin (Hb), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and leukocyte counts (WBC) were determined. The highest of hematocrit was obtained in treatment of temperature of 18oC where the Common carp juvenile were transported in plastic bags with salinity of 5 g/l in water and had significantly difference with the beginning of experiment (P<0.05). Results of hematological analyses are presented in Table 1. Statistical analysis showed significant increase (P<0.05) in values of WBC with the highest values recorded in treatment of temperature-18oC and the lowest values were recorded in plasma of carp at the beginning of experiment and non-significantly (Duncan’s test) in among of another treatments (P>0.05).

کلیدواژه ها:

hematological factors ، transportation ، salinity ، erythrocyte ، hemoglobin concentration