The quality and purity of the proteins extracted from the Lathyrus sativus seeds and optimization by the response surface methodology (RSM)

Lathyrus sativus Linn. (chickling vetch or grass pea)seeds could be valuable sources of protein, Protein constitutes ٢٥.٦-٢٨.٤% of the seed dry weight. It is cultivated widely in every part of Iran but scarce studies about their protein isolated production exist. In this research, a series of statistically designed studies (central composite design (CCD) and response surface methodology (RSM)) were performed to investigate the effect of each of the independent variables (time, ٢٠-٨٠ minutes), (water to solid ratio, ١:٥-١:٢٠) and (pH, ٢.٥-١٠) on protein extraction yield and purity. Results showed that extraction time and pH are two effective factors on quality and purity of the protein isolated (p<٠.٠٠١); In the event that quadratic of water to solid ratio is significant at the ٥%. The optimum protein purity was found to be ٩٣.٦% at ٤٣.٣٨ min, pH ٢.٥٧ and water: solid ratios of ٩.٩١. Results of this research showed a good performance of response surface methodology in the optimization of this process.

کلیدواژه ها:

Lathyrus sativus ، optimization ، protein purity ، response surface methodology