Morphological and Viability Study of WJ-Stem Cells on Different Nano-Bioactive Ceramics for Bone Tissue Engineering Application

Today, bioceramics are widely used in bone tissue engineering which implies cells and scaffold interactions. Herein, we isolated Wharton's Jelly Mesenchymal Stem Cells (WJ-MSCs) from cord tissue of the infants delivered by cesarean section and cultured on four types of Nano-Bioactive ceramics: SiC, SiO2, Calcified Hydroxyapatite (CHAp) and Merck Hydroxyapatite (MHAp). For characterization of derived WJ-MSCs, the Flowcytometery analysis expressed CD105, CD106, CD44, CD34 and CD144 markers. Proliferation of cultured WJ-MSCs observed under Fluorescence Microscopy with DAPI staining. The viability of WJ-MSCs assayed by Neutral Red and analysed with ANOVA and LSD. The viability or proliferation of the cells cultured on SiO2 and SiC showed a significant increase compared to the control cells cultured on the culture dishes. The morphology and attachment of WJ-MSCs were confirmed by Scanning Electron Microscopy (SEM) imaging. The cells were cultured on CHAp showed an epithelioid and those cultured on MHAp and SiC showed round shape morphology. The cells cultured on SiO2 formed cell aggregate along with the isolated cells scattered throughout this ceramic. In conclusion, the cells could grow on all bioactive ceramics; however, the morphology and the best proliferation rate were belonged to SiO2 and SiC.

کلیدواژه ها:

Nanoparticles ، Bioceramics ، Cell viability ، Cell attachment ، Mesenchymal Stem Cells