Association Study of the miR196a2 Polymorphism with the Risk of Idiopathic Recurrent Pregnancy Loss

Background: MicroRNAs (miRNAs) are short non-coding RNAs, which control gene expression by binding to complementary pieces present in the 3 UTR of the mRNAs of protein coding genes. MiRNAs play very important roles in maintaining normal human body physiology conditions, meanwhile, abnormal miRNA expressions have been found related to many human diseases spanning from psychiatric disorders to malignant tumors. Single nucleotide polymorphisms (SNPs) within precursor microRNAs (miRNAs) can disturb miRNAs expression, and may be complicated in the pathogenesis of recurrent pregnancy loss (RPL). Recently, developing reports have indicated that disturbed miRNAs expression and genomic mutations contributed to the pathogenesis of recurrent pregnancy loss (RPL). This study aimed to investigate potential associations between the precursor miRNA SNP miR-196a2 T > C and susceptibility to RPL.Methods: we analyzed miR-196a2 T> C polymorphisms by RFLP technique in 100 IRAN, Tabriz RPL patients that suffer RPL. The allele and genotype frequencies for all populations were analyzed. Linkage disequilibrium was performed.Result: Data analysis of the RPL and non-RPL groups for miR-196a2 T> C polymorphism showed no significant association between the groups (p= 0.904; OR = 0.97; 95% CI, 0.61-1.32).Conclusion: These data suggested that T> C mutation in pri-miR-196a2 coding region doesn t contribute to the genetic predisposition to RPL.

کلیدواژه ها:

miR-196a2 ، Recurrent pregnancy loss ، Single-nucleotide polymorphism ، MicroRNAs