Comparison of the Effect of Metronidazole, Tinidazole, Mango and Blueberry Extracts on Trichomonas vaginalis in Vitro

Background: Trichomoniasis is the most common nonviral sexually transmitted human disease that is caused by protozoan Trichomonas vaginalis. Metronidazole is the selective drug in trichomoniasis treatment. However, the reported cases show an increasing trend of drugresistance. This study aimed to evaluate the effect of mango and blueberry extracts on T. vaginalis. Materials and Methods: T. vaginalis was cultured axenically in TYM (Trypticase Yeast Extract) medium supplemented with 10% bovine serum. The effect of mango and blueberry extracts at 50, 100, 200, 400, 800 and 1000 μg.mL-1 on T. vaginalis was studied after 24 and 48 hours. The final numbers of parasite with a hemocytometer and Trypan blue were recorded. Then the value of IC50 [Half maximal inhibitory concentration] and the lethal percent were calculated. In the present study, the metronidazole was used as positive control. The IC50 value of metronidazole and tinidazole were calculated in the concentrations of 0.02, 0.04, 0.08, 0.16 and 0.32 μg.mL-1. Results: The final results confirmed the significant effect of all mango and blueberry extracts concentrations on the reduction of parasite numbers (P-value<0.05(. The extract concentrations of 1000 μg.mL-1 had the most significant effect on T. vaginalis growth inhibition after 24 hours. The IC50 values of mango and blueberry extracts, metronidazole, and tinidazole were calculated as 118.3, 60.74, 0.042 and 0.02 μg.ml-1 respectively. Conclusion: Based on the obtained results, the different concentrations of mango and blueberry extracts have significant anti Trichomonas vaginalis activities. It is suggested carrying out further studies on suitable animal models.

کلیدواژه ها:

Trichomonas vaginalis ، Mangifera ، Blueberry plant ، Metronidazole ، Tinidazole ، Inhibitory concentration 50