Detection of icaABCD Genes and Biofilm Formation in Clinical Isolates of Methicillin Resistant Staphylococcus aureus
محل انتشار: فصلنامه آسیب شناسی ایران، دوره: 9، شماره: 4
سال انتشار: 1393
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 1,074
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شناسه ملی سند علمی:
JR_IJP-9-4_004
تاریخ نمایه سازی: 18 اسفند 1393
چکیده مقاله:
Background & Objectives: Methicillin resistance Staphylococcus aureus (MRSA) is one of the most important pathogens that causes several nosocomial and community infections. Adhesion to surfaces and biofilm formation is considered main step in staphylococcal infection. The aims of this study were to determine presence of icaABCD genes and relation to the biofilm formation in of MRSA isolates.Methods: Of the 63 MRSA clinical isolates collected from selected hospitals in Tehran, Iran,quantitative biofilm formation was determined by microtiter tissue culture plates (Mtp). All MRSA isolates were examined for determination the icaABCD genes by using PCR method.Results: twenty nine (46%) of the isolates were strong produced biofilm on Mtp. All of the MRSA isolates carried icaD and icaC genes, whereas, the prevalence of icaA and icaB was 60.3% and 51% respectively.Conclusions: S. aureus clinical isolates have different capacity to production biofilm and adhesion. This may be caused by a different in the expression of biofilm genes and hetrogenicity in genetic origins.
کلیدواژه ها:
نویسندگان
Mohsen Mirzaee
Dept. of Laboratory Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran
Shahin Najar Peerayeh
Dept. of Bacteriology, Faculty of Medical Sciences,TarbiatModares University, Tehran, Iran
Abdol-Majid Ghasemian
Dept. of Bacteriology, Faculty of Medical Sciences,TarbiatModares University, Tehran, Iran