DNA flow cytometric analysis in variable types of hydropic placentas
محل انتشار: مجله طب تولید مثل ایران، دوره: 13، شماره: 5
سال انتشار: 1394
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 519
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شناسه ملی سند علمی:
JR_IJRM-13-5_003
تاریخ نمایه سازی: 16 شهریور 1395
چکیده مقاله:
Background: Differential diagnosis between complete hydatidiform mole, partial hydatidiform mole and hydropic abortion, known as hydropic placentas is still a challenge for pathologists but it is very important for patient management. Objective: We analyzed the nuclear DNA content of various types of hydropic placentas by flowcytometry. Materials and Methods: DNA ploidy analysis was performed in 20 non-molar (hydropic and non-hydropic spontaneous abortions) and 20 molar (complete and partial moles), formalin-fixed, paraffin-embedded tissue samples by flow cytometry. The criteria for selection were based on the histopathologic diagnosis. Results: Of 10 cases histologically diagnosed as complete hydatiform mole, 9 cases yielded diploid histograms, and 1 case was tetraploid. Of 10 partial hydatidiform moles, 8 were triploid and 2 were diploid. All of 20 cases diagnosed as spontaneous abortions (hydropic and non-hydropic) yielded diploid histograms. Conclusion: These findings signify the importance of the combined use of conventional histology and ploidy analysis in the differential diagnosis of complete hydatidiform mole, partial hydatidiform mole and hydropic abortion
کلیدواژه ها:
نویسندگان
Fatemeh Atabaki pasdar
Department of Anatomical Sciences, Urmia University of Medical Sciences, Urmia, Iran
Alireza Khooei
Department of Pathology, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
Alireza Fazel
Department of Anatomy and Cell Biology, Mashhad University of Medical Sciences, Mashhad, Iran.
Maryam Rastin
Immunology Research Center, BuAli Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran