Anti-cancer effect of ICD-85(venom derived peptides) onMDA-MB231 cell line (in vitro) and experimental mice withbreast cancer (in vivo)

Breast cancer has become common in developing and developed countries. Alarming increasein this disease as a leading cause of death in women is a concern of all. Despite the significantimprovements in the management of breast cancer, the survival rate is not more than 20% - 25%. Forthis study, MDA-MB231 cell line was used and the effect of ICD-85 (biologically active peptides fromvenomous animals) was assayed by measuring the activity of the cytosolic enzyme lactatedehydrogenase(LDH) released into the culture medium after membrane legions. Morphologicalchanges of cells were checked in control and cells incubated with ICD-85 as chemo preventive agent.Results showing in test groups - incubation with 10μg/ml dose of ICD-85 had decreased cytoplasmicbranch. Some cells were ruptured and lost the continuity of their surrounding membranes, and numberof cells was decreased. On the other hand, when ICD-85 was used as an anticancer treatment of micewith breast cancer, it was shown that ICD-85 can prevent the growth of breast tumor, increase the lifeduration of experimental mice and inhibit angiogenesis in breast tumor as compared with controlgroup. It seems that ICD-85 acts at the membrane level and can prevent the cell growth as well asproducing apoptosis (cell suicide) for breast cancer cells.

کلیدواژه ها:

anticancer ، breast cancer ، ICD-85 ، mice