The inhibitory effect of silver nanoparticles on the bacterial fishpathogens, , ,andStreptococcus iniae Lactococcus garvieae Yersiniaruckeri Aeromonas hydrophila

The aim of this study was to assess the antibacterial effects of silver nanoparticles (Nanocid®) on 30 isolates of Gram-positive and Gram-negative bacteria that are pathogenic to fish, which consisted of strains of Streptococcus iniae Lactococcus garvieae Yersinia ruckeri and Aeromonas hydrophila Aeromonas hydrophila after the inoculation of these bacteria. Isolates were prepared in sterile phosphate buffered after the inoculation of these bacteria. Isolates were prepared in sterile phosphate buffered isolates) the results showed that the MBCs were in a range from more than 10 μg/ml to 0.62 μg/ml 30-90 minutes post-inoculation. Also, the range of MBCs for was 0.31 μg/ml to less than 0.15 μg/ml, while the range of MBCs for was from 2.5 to 0.62 μg/ml 30 to 90 minutes post-inoculation, respectively.Generally, the bacterial growth for these bacterial species was reduced from 1 × 10 cells/ml to no growth compared to initial inoculumsof 10 cells/ml during 90 minutes after exposure to silver nanoparticles. No remarkable change was found in the ba cterial count for the control samples (PBS without silver nanoparticles) during the experim

کلیدواژه ها:

silver nanoparticles ، streptococus ، iniae ، lactococcus gavieae ، yersinia ruckeri ، Aeromonas hydrophila ، MBC