The symbiotic effect of Piriformospora indica on induced resistance againstbakanae disease in rice (Oryza sativa L.)

The root endophytic fungus, Piriformospora indica, colonizes roots of a large number of plant speciesincluding Cereals and Brasicaceae. There are several reports indicating that P. indica protects roots from different pathogens. In the present study, rice plants were pre-inoculated with P. indica and were subsequently infected with Fusariumproliferatum, as the causal agent of root rot and crown rot (Bakanae) disease. The effects of P. indica on the protectionof rice plants against F. proliferatum were evaluated. Transcription levels of some pathogenesis-related genes such asNPR1, PR1, PR4 and PR5, as well as two transcriptional factor genes namely WRKY62 and WRKY85 were quantifiedvia quantitative real time PCR in rice roots. Pre-inoculated and challenged rice plants showed mild disease symptomswith two weeks delay compared to non P. indica colonized plants. Quantification of Fusarium in roots via q-PCR confirmed the higher quantity of the fungi in non pre-inoculated plants. Expression analysis revealed a faster induction ofpathogenesis related genes following F. proliferatum inoculation of pre-inoculated plants i.e. priming of these genes is apart of mechanism of P. indica induced systemic resistance.

کلیدواژه ها:

Priming ، q-PCR ، Timing ، Symbiosis