Topical gel formulation and stability assessment of platelet lysate based on turbidimetric method

سال انتشار: 1394
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 578

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شناسه ملی سند علمی:

JR_TIPS-1-2_003

تاریخ نمایه سازی: 17 فروردین 1395

چکیده مقاله:

Platelet-rich growth factors have attracted attentions of scientists and clinical practitioners who are involved in wound healing and regenerative medicine extensively, according to their unprecedented potential of promoting and catalyzing healing process. Platelet-rich growth factors are cost-benefit, availableand more stable than recombinant human growth factors. These appealing characteristics have converted PRGF to one of the popular candidates for treatment of variety of wounds. According to these valuable properties, we decided to formulate and assess the effect of different excipients on the stability of suchvaluable protein based formulations. Different excipients have been chosen according to their effectiveness on the stability of proteins and their application in other similar formulations. The stabilizing effect of excipients was evaluated by measuring heat-induced aggregation of growth factors by turbidimetric assay.Glycerol, glycine and dextrose were chosen as stabilizing excipients for these formulations. The resultsshow that dextrose has more stabilizing effect on prevention of heat induced aggregation of the platelet lysate growth factors than glycerol and glycine. All of the formulations also contained antioxidant, chelating agents, preservative and carbopol934 in order to form appropriate gel.

نویسندگان

Soliman Mohammadi Samani

Center for Drug Delivery in Nanotechnology, School of Pharmacy, Shiraz University of Medical Sciences,Shiraz, Iran

Akram Jamshidzade

Pharmaceutical Sciences Research Center, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran

Seyed Modjtaba Seyed Raoufi

School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran

Zeinab Mardani

School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran