THE PREVALENCE OF GYRA GENE MUTATIONS IN FLUOROQUINOLONES-RESISTANT PSEUDOMONAS AERUGINOSA ISOLATES IN GUILAN PROVINCE

Background and Aim:Pseudomonas aeruginosa is one of the most common causes of opportunistic infections. There are several mechanisms for resistance to antibiotics in this bacterium. Fluoroquinolones are the only accessible antibiotics for effective oral treatment of infections caused by this organism. Among fluoroquinolones, ciprofloxacin is widely used in the treatment of P. aeruginosa infections. The aim of this study was to evaluate the prevalence of gyrA mutations in fluoroquinolones-resistant P. aeruginosa isolates in Guilan Province.Methods:In this study, a total of 68 clinical isolates of P. aeruginosa were collected. All isolates were identified by standard biochemical tests. The antibiotics susceptibility was determined by disk diffusion method and MIC; Then, PCR-sequencing was carried out to identification mutation involved in fluoroquinolones resistance.Results:From 68 clinical isolates of P. aeruginosa, 12 isolates were fluoroquinolone resistance. Ciprofloxacin resistant isolates showed MIC between 32 to 1024 μg/ml. PCR-sequencing showed all 12 fluoroquinolone resistance isolates had at least one or more mutation in gyrA gene.Conclusion:The results of this study showed that the rate of resistance to ciprofloxacin is significantly increasing, which may be due to various mutations in the gyrA gene. Early detection and infection-control are the best antimicrobial strategies for this organism.

کلیدواژه ها:

Pseudomonas aeruginosa ، Ciprofloxacin ، gyrA ، mutation