PRODUCTION OF COML RECOMBINANT AND ITS FUNCTIONAL ROLE IN ANTIBIOTIC RESISTANCE

Background and Aim:Resistance to antimicrobial drugs in the treatment of infectious diseases causes problems. The absorbent protein of the DNA, ComL, is one of the proteins involved in the transformation of resistance plasmid. This protein is located on the outer membrane of Acinetobacter baumannii. In this study the immunogenicity of ComL protein and its role in absorbing antibiotic-resistant plasmids was investigated.Methods:Coml gene in A. baumannii ATCC19606 genome was amplified, cloned in pET28a vector and the recombinant construct was transformed into E.coli BL21 (DE3). The recombinant protein was purified by Ni-NTA chromatography column and injected into BALB/C mice for immunization.Results:The Kanamycin sensitive and to ampicillin resistant A. baumannii ATCC19606 was exposed to immune serum of mice for 1 hour showed resistance to Kanamycin In vitro, and reduction of about 60% in CFUs. A. baumannii were prepared as described above and injected to non-immunized mice at lower than LD50. The number of Kanamycin -resistant bacteria colonized in the spleen was 30% lower than that of control in the Kanamycin-ampicillin medium. The number of bacteria colonized in the spleen of the immune mice was been observed to be 3 fold lower than the control.Conclusion:Antibodies against the 22KD ComL could prevent A. baumannii resistance to antibiotic, is an option for infection treatment.

کلیدواژه ها:

ComL protein ، A. baumannii ، Antibiotic –resistance ، Recombinant Protein