DESIGNING A NANOBIOSENSOR FOR DETECTION OF BRUCELLA ABORTUS BASED ON POLYCLONAL ANTIBODY AND SILICA NANOPARTICLES

Background and Aim:Brucellosis is caused by the livestock, meat and dairy products contaminated with Brucella spp. Currently, there are several methods for the diagnosis of Brucella spp., but none of them have enough sensitivity and specificity. Therefore, providing a new diagnostic way with high performance seems to be necessary. In this study, detection of Brucella abortus was investigated based on polyclonal antibody against Brucella abortus and Blue silica.Methods:Initially, blue silica nanoparticles were synthesized. Then, it was conjugated to the polyclonal antibody. After functionalization, the nanoparticles were added to the microbial suspension containing Brucella abortus. Subsequently, dye was to be released from the complex. Finally, the sensitivity and the specificity of nanobiosensor were investigated in various concentrations of Brucella abortus and other bacterial strains such as E.coli, Salmonella, Shigella, Staph aureus and Pseudomonas, respectively.Results:Based on the results, sensitivity was determined 1.5 × 103 CFU mL-1. Also, specificity was confirmed in the presence of other bacteria.Conclusion:The results of this study showed that, this technique has a high ability to fast detection of brucellae.

کلیدواژه ها:

Brucella abortus ، Detection ، Silica nanoparticles ، polyclonal antibody