IN SILICO DESIGNING AND ANALYSIS OF A CHIMERIC VACCINE AGAINST CLOSTRIDIUM PERFRINGENS AND EIMERIA SPP

Background and Aim:In poultry, necrotic enteritis (NE) is caused mainly by α toxin and the pore-forming toxin NetB of Clostridium perfringens. The ability of the bacterium to cause disease is linked to several predisposing factors that affect intestinal conditions and create a favorable environment for proliferation of bacteria. Perhaps the most important of these factors is the incidence of coccidiosis. NE incidence and the mortality rates are higher when chickens are co-infected with Eimeria (coccidiosis). The aim of this research is in silico design a bivalent vaccine against both diseases.Methods:Amino-acid sequences of NetB, α toxin, and AMA1 (from Eimeria) were obtained from UniProt. To determine their antigenic potency, solubility and localization of proteins were analyzed separately and together as a chimer, using VAXIJEN, PROSO II, and TMHMM databases. The recombinant gene was optimized with codon optimization tools for expression in prokaryotic systems. The mRNA structure and stability were analyzed. The stability and 3-D structure of the chimeric protein (using ITASSAR) with an appropriate linker was also determined.Results:According to our in silico analyses, the chimeric protein was stable with high antigenicity and immunogenicity.Conclusion:In conclusion, this stable immunogenic construct could generate a potent immune response against Clostridium perfringens and Eimeria spp and may be considered as a bivalent NE and coccidiosis vaccine candidate for in vitro studies.

کلیدواژه ها:

necrotic enteritis ، coccidiosis ، Clostridium perfringens ، Eimeria ، bioinformatics