PHYLOGENIC ANALYSIS OF HUMAN BOCAVIRUS IN CHILDREN WITH ACUTE RESPIRATORY INFECTION IN IRAN

Background and Aim:Human bocavirus (HBoV) was first described in nasopharyngeal aspirates samples from children that suffer from respiratory illness in 2005. This virus has possibility role in acute respiratory infections as well as gastroenteritis in children. This study was performed to analyze the infection frequency and confection rates of HBoV with RSV and phylogenetic analysis of the virus in children with acute respiratory infection in Iran.Methods:During the time period 2016 to 2017, a total of 75 respiratory samples from children hospitalized with acute respiratory infection were collected. The samples were first screened for RSV by direct immunofluorescence method and then subjected to detect HBoV by PCR with primers from the NP-1 gene.Results:The isolated HBoV were sequenced for VP2. From 75 respiratory samples, 20(26.7%) and 10 (13.3%) were positive for RSV and HBoV, respectively. The coinfection rate was 40 % (P <0.05). In seasonal distribution, the winter has upmost extent outbreak (P < 0.05). Sequence analysis of positive samples revealed that all of them are related to HBoV 1 genotype.Conclusion:This study demonstrated that detection of the virus in clinical samples from children with acute respiratory infection was low, and all of the detected viruses were related to HBoV genotype.

کلیدواژه ها:

Human bocavirus (HBoV) ، acute respiratory tract (ARTI) infection ، RSV ، Phylogenetic analysis