ENHANCMENT OF CD22 EXPRESSION PROFILE IN ESCHERICHIA COLI ROSSETA (DE3) BY EXPOSURE TO EXTREMELY LOW FREQUENCY MAGNETIC FIELDS

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 429

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شناسه ملی سند علمی:

MEDISM19_576

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:Over the last two decades, application of bacterial host cells, especially Escherichia coli (E. coli), for heterologous expression of proteins have gained much attention, mainly due to their ease of genetic manipulation, rapid growth and low cost media. Alongside, many studies have reported the significant biological effects of non-thermal extremely low frequency magnetic field (ELF-MF) produced by an alternating current (AC) on bacteria, proposing the possibility of improving expression profile of proteins by further applying magnetic fields in these host cells.Methods:In present work, the expression profile of cluster of differentiation-22 (CD22) as a eukaryotic membrane protein in E. Coli Rosetta (DE3) was examined in the presence of ELF-MF. Different concentrations of isopropyl-b-D-thiogalactopyranoside (IPTG) (0.25, 0.5, 0.75 and 1 M) were applied as inducer and expression was carried out completely in the presence of ELF-MF. Depending on the extend of applied IPTG, an electromagnetic fields (EMFs) with an strength equal to 55 mT and the frequency range of 2.5-2.8 HzResults:a significant enhancement in protein expression levels. Nevertheless, EMFs with intensities rather than 55 mT resulted a significant decrease in protein expression levels when the IPTG concentrations were equal to 1 M or 1.25 M. In conclusion, current study, demonstrated that exposing E.coli to ELF-MF within a specific narrow frequency window can bring about significant enhancement in protein expression.Conclusion:exposure to non-thermal ELF-MF within a specific narrow frequency window can significantly improve heterologous expression of CD22 as a Eukaryotic membrane protein in E. coli.

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نویسندگان

Behnaz Rashidieh

Department of Microbiology, Faculty of Advanced Sciences & Technology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran