GENOTYPING OF MYCOPLASMA AGALACTIAE ISOLATED FROM SHEEP IN ARDABIL AND GOLESTAN PROVINCES OF IRAN COUNTRY

Background and Aim:Currently, Multilocus Sequence Typing (MLST) is one of the new molecular techniques of bacterial typing which is based on the investigation of allelic differences in the sequence of housekeeping genes. Mycoplasma agalactiae is the etiologic agent of contagious agalactia of sheep and goats, a disease involving acute mastitis, arthritis, keratoconjunctivitis, and abortion when first introduced in a susceptible population. The aim of this study was molecular typing of M.agalactiae isolates was done throughout MLST the most reliable typing method, which is based on DNA sequencing.Methods:The MLST typing system targeting 5 house keeping genes of dnaA ,gltX ,gyrB ,metS and tufA with few modifications was performed on strains. The amplification products were sequenced to guaranty accuracy of sizing and nucleotide structure of unit repeats. The Sequence Type (ST) and the corresponding clonal complex of the strains was determined by consulting the MLST database.Results:One categorically different sequence types (ST) identified in this study with one new allele found at gyrB gene has not been reported before.Conclusion:Detection of one ST type among two isolates from different geographical locations reflects a low level of diversity in the M.agalactiae population. We have a belief that still a lot needs to be done at the national level so that the epidemiology of M.agalactiae in Iran is fully acknowledged

کلیدواژه ها:

housekeeping genes ، agalactia ، Mycoplasma agalactiae ، allele ، MLST database