THE EVALUATION OF IMMUNITY OF INACTIVATED RAZI BLACKLEG VACCINE BY AN INDIRECT ELISA AND COMPARISON OF THE METHOD WITH CHALLENGE TEST

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 405

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شناسه ملی سند علمی:

MEDISM19_642

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:Blackleg is an acute infectious disease caused by Clostridium chauvoei and is characterized by inflammation of muscles, severe toxemia. The main purpose of this study was the evaluation of immunity of blackleg vaccine by using an indirect ELISA system and comparison of the method with challenge.Methods:In this case, after preparation of whole cell antigens of Clostridium chauvoei, positive and negative sera were prepared by injection of rabbits, the blood samples were collected on 0, 15, 28 days after first vaccination and on 15, 45, 90, 180, 270, 360 days after second vaccination. An indirect ELISA test was carried out for detection of antibodies against Clostridium chauvoei and then the results were compared with the challenge assay in which animals were inoculated with Clostridium chauvoei.Results:The results showed the rabbit ELISA titers remained up to on 270 days after second vaccination had a cut off titer 305.6 Unit/ml (100% protection), whereas on 360 days showed considerable decrease in rabbit ELISA titers. This experiment was confirmed by the challenge assay on guinea pigs. It was showed that correction coefficient (r=0.74) was significant between an ELISA titers and challenge assay and also there was a significant variation by t-test (t=1.376, P<0.05) between two groups of vaccinated (test) and unvaccinated animals(control).Conclusion:It could be concluded that the ELISA is a proper test for evaluation of immune status of rabbit and has a significant correlation with challenge test and also ELISA method can use as an alternative for challenge test.

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نویسندگان

Ali Haghroosta

Department of Anaerobic Bacterial Vaccines Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization(AREEO), Karaj, Iran

Reza Pilehchian Langroudi

Department of Anaerobic Bacterial Vaccines Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization(AREEO), Karaj, Iran

Lida Abdolmohammadi Khiav

Department of Anaerobic Bacterial Vaccines Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization(AREEO), Karaj, Iran

Mona Hasanzadeh

Department of Anaerobic Bacterial Vaccines Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization(AREEO), Karaj, Iran