ISOLATION AND MOLECULAR IDENTIFICATION OF MYCOBACTERIUM FROM RAINBOW FISHES IN SABZEVAR

Background and Aim:Fish tuberculosis is a common disease between human and aquatic animals. Several species of Mycobacterium cause tuberculosis in fish. It usually appears as a skin disease, but systemic form of the disease has been reported in human and fish. Due to the possibility of this infection in farmed fish and the risk of public health and nutrition in humans, isolation of these species are necessary. The purpose of this research was isolating and molecular identifying of Mycobacterium from rainbow fishes in Sabzevar.Methods:In order to carry out of this research, 50 rainbow fishes were randomly collected from seven swimming pools in separate areas of Sabzevar city. Head, fins and internal organs cultured as routine manner. DNA extracted from positive cultures and PCR-16srRNA were accomplished. The PCR product was sequenced for final confirmation.Results:Out of 50 rainbow fishes, three Acid fast bacteria were isolated. The PCR-16srRNA were positive by specific primers for Mycobacterium. The results of the nucleotide sequencing were analyzed by the BLAST program, which all of these three isolates were identical and similar to Mycobacterium peregrinum.Conclusion:It is possible that, there is similar source for all of these fishes farming. This research suggests that, there is a possibility of transferring pathogenic Mycobacterium to humans.

کلیدواژه ها:

Fish tuberculosis ، Mycobacterium peregrinum ، Rainbow ، Primer