OPTIMIZATION OF SINGLE NUCLEOTIDE POLYMORPHISM (SNP) ANALYSIS IN THE CLASSICAL PCR MACHINES FOR TYPING OF MYCOPLASMA AGALACTIAE

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 426

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شناسه ملی سند علمی:

MEDISM19_686

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:Contagious agalactia is a chronic infections in small ruminants and remains endemic in many regions of the world, despite intensive and costly eradication programs. To prevent the disease, a vaccine containing three strains of Mycoplasma agalactiae namely Taleghan, Lorestan and Shiraz is currently used in Iran. The present study was aimed to optimize SNP analysis on the basis of P40, P48 and P80genes in order to molecular typing of M.agalactiae for the first time.Methods:Primer 3 program was used to design specific primers based on P40, P48 and P80 genes of the organism. M. agalactiae Taleghan, as a standard strain, was obtained from the microbial archive of the Razi Vaccine and Serum Research Institute. A universal PCR protocol was developed to enable simultaneous amplification of all genes by conventional PCR machines.Results:Access of a common PCR protocol, which is able to proceed simultaneously four different reactions in one PCR run, was the primary outcome of this research.Conclusion:contemplation the fact that goat and sheep farming play an important role in the economy of the Iran, so it is recommended this method used for molecular characterization of strains to other provinces and compare them with vaccine strain.

نویسندگان

Khatereh Kabiri

Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran

Keyvan Tadayon

Department of Veterinary Aerobic Bacteria Razi Institute Karaj

Seiyed Ali Pourbakhsh

Mycoplasma reference laboratory, Razi vaccine and serum research institute, Karaj, Iran

Jamile Norouzi

Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran