EFFECT OF SACCHAROMYCES CEREVISIAE ON EXPRESSION TOXIN GENE STX1 IN ENTEROHAEMORRHAGIC ESCHERICHIA COLI EHEC

Background and Aim: Enterohaemorrhagic Escherichia coli (EHEC) is an important pathogen in the world that cause acute disease such as Hemolytic-Uremic Syndrome (HUS).EHEC produces Stx1 that cause this disease. Consumption of Probiotics is a new method to curing some illnesses. Probiotic Saccharomyces cerevisiae can decrease microorganisms or their effects. The aim of this study is to evaluate the effect of Saccharomyces cerevisiae on the expression of the produced toxin gene (Stx1) by EHEC.Methods:Supernatant and lysate of Saccharomyces cerevisiae were prepared so dried. The level of Stx1 toxin genes expression was measured by Real Time technique in the present of concentrations of 1/2 MIC of supernatant and the highest concentrations for lysate.Results:Supernatant and lysate reduced the gene expression by 77% and 61% respectively.Conclusion:The both of Supernatant and Lysate decreased the expression of Stx1 but Supernatant was more effective than Lysate.