Bioinformatic analysis of Antimicrobial peptide derived from Iranian scorpion, Hemiscorpious Lepturus

سال انتشار: 1395
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 784

نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

MPHBS01_129

تاریخ نمایه سازی: 22 آبان 1395

چکیده مقاله:

Introduction: Microbial resistance to antibiotics is a growing concern among the health care experts which has made them searching for the alternative therapies. In recent years, antimicrobial peptides have gained much interest as an alternative to the traditional antibiotics. Antimicrobial peptides naturally exist in all the organisms and play an essential role in their intrinsic immune system. Many of them have been identified in recent decades and separated from insects, Amphibians and mammals. Antimicrobial peptides have vast activities and could be used as antibacterial, antiviral, antifungal and even sometimes as anti-cancer and anti-tumor agent. The researches have shown that each scorpion could possess more than 100 different peptides with weight ranging from 1000-9000 Dalton. Materials and methods: Antimicrobial peptides have low sequential homology. Therefore, by having RNA sequence in venom gland of Hemiscorpious Lepturus, and bio-informatics software, we tried to identify these peptides. First, Hemiscorpious Lepturusvenom was obtained. RNA extracted by standard method and then sequencing of active RNA genes were performed by external service and DNA sequences were received as a databank. After searching for required codes and extracting DNA sequence, the largest ORF of the genes was obtained via Expasy server, for each of them, BLAST tests were performed. After investigating the sequence similarities, the active sites of the main peptide chain was discovered, and 2-d and 3-d structures of the peptide was predicted. Results: Among the codes, only 4 of them were associated with antibacterial peptides. The sequences were translated by EXPASY TRANSLATOR server. For the largest ORF, BLASTP test was done. The results showed that one sequence belongs to antimicrobial peptides super family. After characterization of the similar peptides via different servers, it was revealed that the mentioned peptide is structurally similar to antibacterial peptide of South American and African scorpion venom. Conclusion: Characterization of these peptides could be used for tracing a proper therapeutic potential agent.

نویسندگان

Akram Kouhi

M.Sc. Student of Toxicology, Department of Pharmacology-Toxicology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, (IAUPS), Tehran, Iran.

Zahra Mousavi

Associate Professor, Department of Pharmacology-Toxicology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, (IAUPS), Tehran, Iran

Kamran Pooshang Bagheri3

Pasteur Institute of Iran, Biotechnology Research Center, Biotechnology Dept. Tehran, Iran.