The construction of a novel proinsulin with mini C – peptide for development of recombinant insulin production

To increase the folding efficiency of the proinsulin and the production yield of insulin, we have displaced C- peptide of proinsulin with the sequence EAEDLQ. The instability index of mini- proinsulin and fusion part is computed to be 26.38. This classifies the protein as stable. The mini-proinsulin was fused at the N-terminus to thioredoxin fusion partner containing a His6 tag for affinity purification. The gene for the fusion protein was inserted downstream of the T7 promoter of the expression plasmid pET-32a. The observed results from native PAGE, ELISA and HPLC show improvement in recombinant insulin production

کلیدواژه ها:

Mini proinsulin ، recombinant ، origami B