Development of Dispersive Liquid–Liquid Microextraction Technique based Low-Density Solvents for the Extraction and Preconcentration of Amoxicillin Antibiotic

Amoxicillin is the most prescribed for the treatment infections worldwide. Low-density solvents have been used largely in analytical extraction and concentration schemes. Their ability to form a phase with regions of different polarities, acidities and viscosities, where solutes of very different structure can besolubilized has encouraged this use. supramolecular solvents made up of reverse micelles of decanoic acid, dispersed in a continuous phase of THF-Water, was proposedfor the simple, fast and efficient microextraction of amoxicillin prior to high performance liquid chromatography (HPLCUV).The extraction method is based on the fast injection of a mixture of extracting and disperser solvents into the aqueous solution toform a cloudy ternary component solvent (aqueous solution:extr acting solvent:disperser solvent) system. The extraction procedure isbased on extraction solvents lighter than waterand performing of extraction in a specially designed extraction cell. Our attempt were centered on investigating factors affecting theextraction efficiency of amoxicillin such as pH of water sample, percent of THF, percent of decanoic acid, microextraction time andtemperature effect were investigated. The method was validated for specificity, linearity, accuracy, precision and limit of detection. Inthis extraction method, under the optimum conditions, a correlation coefficient of r2 = 0.999 was obtained. The LODs were low of0.011 μg L-1 and RSD% ranging from 1-4%.Finally, duo to simplicity and high efficiency of proposed method, it can be used successfully for manufacturing process,quality control in product batches, preconcentration and determination of amoxicillin.

کلیدواژه ها:

Amoxicillin ، DLLME ، Low-density solvents ، HPLC