Diagnosis and Treatment Acute Lymphoblastic Leukemia with Bioinformatics Approaches

Acute lymphoblastic leukemia is caused by damage to DNA that leads to uncontrolled cellular growth. Herein, gene differential expressions, epigenetic and protein analysis of classic Hodgkin and acute lymphoblastic leukemia analyzed by various bioinformatics tools to investigate new diagnostic and treatment approaches.Method: Microarray libraries GSE20011 downloaded from NCBI database and analyzed with GEO2R software, then differential expression genes analyzed by four databases (DAVID,Wikipathways, BioCarta and KEGG databases). Protein kinases analysis, transcription factor analysis, protein-protein interaction network and microRNA analysis performed by X2K ,ChEA, Genes2Networks,TargetScan microRNA software respectively. Finally, drug target identification and Histone modification factor carried out by Drug Pair Seeker and ENCODE databases.Results: The results showed the highest SUZ12 Transcription Factor (TF) binding to up-regulated genes while it was Sox2 for down-regulated genes. Functional analysis of up-regulated genes showed highly activation in Plasma membrane while Cytokine receptor activity and Cytokine binding noted in down-regulated genes. Protein –protein interaction networks and drug discovery facilities revealed that Cephaeline drug induces down-regulated genes while Lycorine represses up-regulated gene networks. The microRNA-501 repressed up-regulated genes. The finding paves the roads toward acute lymphoblastic leukemia therapy with miR-501 and Lycorine/Cephaeline drugs.

کلیدواژه ها:

acute lymphoblastic leukemia ، MicroRNA ، Treatment ، Bioinformatics tools