Design, Synthesis and cytotoxicity studies of oxali-palladium nanoparticles using ginger extract

Introduction: Green nanobiotechnology means the synthesis of nanoparticles or nanomaterials using Routes biological such as microorganism, plants and viruses which among them plants are the best candidates. Ginger includes several biomolecules such as alkaloids, flavonoids and terpenoids which have represented a wide range of pharmacological activities (antibacterial, antiviral, anti inflammatory, anti allergic, anticancer and antioxidant). Recently Palladium (II) complexes are considered as anti-tumor drugs due to their structural similarities between Palladium and Platin.Aims: In this study, synthesis, characterization and anticancer properties of oxali-palladium nanoparticles synthesized using ginger root extract against human cancolorectal cancer cell line of HCT116were investigated.Materials and Methods: Extraction of the ginger root in water was prepared and incubated with oxali-palladium solution in a shaker incubator at 50 C for 24 h incubation time.Then, the physicochemical characteristics of oxali-palladium nanoparticles were studied using Dynamic Light System (DLS) and FE-SEM electron microscope. Finally, the cytotoxicity of nanoparticles against colon cancer cells HCT116 was studied using MTT assay. Results: UV-Visible results have shown the alteration in colour of synthesized nanoparticles solution using green chemistry after 24h incubation time, which is indicating of reduction of palladium ions by ginger extract in good condition. In addition, the results of DLS indicated theat synthesized nanoparticles have a size of approximately 85nm and polydispersity of 0.26. In order to confirm the morphology and size of nanoparticles, the Electron microscopy FE-SEM was done that representedthe nanoparticles with morphology of spherical and homogeneous have been synthesized. Finally, the cytotoxicity of nanoparticles against colon cancer cells of HCT116 indicated dose and time-dependent response to induce death in colon cancer cells.Conclusion: According to above results, it can be concluded that the synthesized nano-oxali-palladium using ginger extract has been at the nanoscale, spherical morphology and homogeneous, due to lake of use of any chemical agents in itssynthesis process can be considered as a candidate for the treatment of colon cancer in future

کلیدواژه ها:

Green Chemistry ، Extract Ginger ، Nanooxali-Palladium ، Colon Cancer