THE IMPORTANCE OF FNDC5 ON ENERGY EXPENDITURE AND METABOLISM WITH IMPACT ON NEURAL DIFFERENTIATION

FNDC5 (Fibronectin Type III Domain Containing 5) which identified to be a mediator of adipocyte differentiation has attracted attention due to its specific role in connection between bodily exercise and the cognitive function of brain. To our knowledge, particular endurance exercise has beneficial effects on brain health and cognitive function specially to improve neurological disease states like Alzheimer’s disease. We have shown an increase in the expression of Fndc5 after retinoic acid treatment during the process of neural differentiation. There by using short hairpin RNA (shRNA) encoding vector we performed the knockdown of Fndc5 in neural differentiation of mouse embryonic stem cells. Data have shown that decreased Fndc5 expression significantly reduced expression of neural precursor cells and mature neuronal markers which modulated neuronal differentiation concluding that Fndc5 expression is required for the appropriate neural differentiation. These data confirm the importance of Fndc5 in the generation and development of the nervous system.

کلیدواژه ها:

Fndc5 ، Neural differentiation ، shRNA ، Neural differentiation