Effect of Operational Variables on Aqueous enzymatic oil extraction from soybean

In contrast to traditional extraction methods, aqueous enzymatic extraction of oil from oilseeds is a recent clean technology. This paper reports work performed at laboratory scale to extract soybean oil by aqueous enzymatic extraction method. In the present work the influence of enzymes concentration, extraction time, dilution ratio, particle size and 3 interactions in the final yield are evaluated and process parameters are optimized by means of taguchi method.48 experiments of extraction carried through and statistical analysis showed that particle size was the most significant variable on oil extraction. Soybean oil was extracted under optimized aqueous extraction condition using 3 enzymes; dilution: 7:1, agition rate: 220rpm, size: 355mic, time of enzymatic operation 1 and 12 hr. The maximal oil extraction yield at optimum condition was 61.42%.

کلیدواژه ها:

Aqueous enzymatic extraction ، Cellulase soybean oil ، Pectinase ، Protease ، Taguchi method