Arbutin Administration Attenuates Memory Deficit and Neuronal Loss in Pentylenetetrazol-Induced Kindling Model in Mice

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 557

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شناسه ملی سند علمی:

NSMED01_044

تاریخ نمایه سازی: 5 آذر 1397

چکیده مقاله:

Epilepsy is one of the most common chronic neurological disorders which provoke progressive neuronal degeneration and memory impairment. In recent years, application of herbal compounds with anti-inflammatory properties, such as arbutin has been introduced as useful agent in reducing of the epilepsy symptoms. In this study, the effect of arbutin application on memory improvement and neuronal density was evaluated in pentylenetetrazol (PTZ)-induced kindling model. Male NMRI mice have received the daily injection of arbutin at dose of 25 and 50 mg/kg. All interventions were injected intraperitoneally (i.p.), 10 days before PTZ administration and the injections were continued until 1 h before each PTZ injection. Spatial learning and memory was evaluated using Morris water maze test after the 7th PTZ injection. Animals have received 10 injections of PTZ and then, brain tissues were removed. Immunostaining against NeuN as mature neuronal marker was performed on brain sections. Our results showed that arbutin administration reduced the seizures behavior and prevented cognitive impairment in fully-kindled animals. Immunostaining data demonstrated that the level of neuronal loss was reduced in animals under treatment of arbutin. Overall, the results of this study suggest that arbutin administration effectively ameliorates memory impairment and alleviates the level of neuronal death in PTZ-induced kindling model.

نویسندگان

Seyed Raheleh Ahmadian

Student Research Committee, Babol University of Medical Sciences, Babol, Iran

Mahdi pouramir

Cellular and Molecular Biology Research Institute, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran

Maryam ghasemi

Cellular and Molecular Biology Research Center, Health Research Center, Babol University of Medical Sciences, Babol, Iran

Farzin shadghi

Cellular and Molecular Biology Research Center, Health Research Center, Babol University of Medical Sciences, Babol, Iran