Influence of Collagen on Characteristics of Enzymatically-Gellable Pectin Hydrogels for Tissue Engineering Applications

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 470

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شناسه ملی سند علمی:

WTRMED05_036

تاریخ نمایه سازی: 5 آذر 1397

چکیده مقاله:

Pectin is a natural polysaccharide that has recently gotten interests in biomedical and biopharmaceutical applications. Collagen, that is another useful macromolecule in tissue engineering applications, was added to the enzymatically-gellable pectin to enhance its adhesive properties and cell interactions due to the lack of adhesive molecules in polysaccharides. Herein, to enrich the other features of pectin such as mechanical strength, it was modified by tyramine hydrochloride in the presence of EDC/NHS for 1 day at 25°C. Then the pectin/ collagen in situ hydrogels (PC) with a different ratio of pectin and collagen were produced. The PC hydrogels of 2.1% pectin/ 0.9% collagen (PC1), 1.5% pectin/ 1.5% collagen (PC2) and 0.9% pectin/ 2.1% collagen (PC3) were prepared and characterized by enzymatic degradation, SEM, mechanical strength, swelling properties and water vapor loss using optimized ratio of horse radish peroxidase (HRP) and H2O2 respectively, 0.1 U/ml and 0.35 mM. Afterwards, fibroblast cells as model stem cells at x_(0=) 1×〖10〗^6 cells/ml gel was seeded for 7 days and MTT assay was done for cell activity measurements. PC1 hydrogels displayed high swelling ratio and further better mechanical performance in comparison with other PC hydrogels. SEM images illustrated high porosity of PC3 hydrogel as compared to other hydrogels containing less collagen. In cell growth analysis, however, PC3 hydrogels revealed about 2 times increase in fibroblast cell proliferation. The study demonstrated the potential of enzymatically-gellable pectin as an economical scaffold for use in tissue engineering applications.

نویسندگان

Mehri Ahmadian

MSc of Chemical Engineering-Biotechnology / Sahand University of Technology / Tabriz / iran

Ali Baradar Khoshfetrat

MSc of Chemical Engineering-Biotechnology / Sahand University of Technology / Tabriz / iran

Neda Khatami

MSc of Chemical Engineering-Biotechnology / Sahand University of Technology / Tabriz / iran

Fatemeh Morshedloo

MSc of Chemical Engineering-Biotechnology / Sahand University of Technology / Tabriz / iran