Zahra Samadi Noshahr - Department of Pharmacology, Zahedan University of Medical Sciences, Zahedan, Iran
Mohammad Reza Shahraki - Department of Physiology, Zahedan University of Medical Sciences, Zahedan, Iran
Hassan Ahmadvand - Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorram Abad, Iran
Davood Nourabadi - Department of Physiology, Iran University of Medical Sciences, Tehran, Iran
Alireza Nakhaei - Department of Pharmacology, Zahedan University of Medical Sciences, Zahedan, Iran

Background: We investigated the effects of Withania somnifera root (WS) on insulin resistance, tumor necrosis factor α (TNF-α), and interleukin-۶ (IL-۶) in fructose-fed rats. Methods: Forty-eight Wistar-Albino male rats were randomly divided into four groups (n=۱۲); Group I as control, Group II as sham-treated with WS by ۶۲.۵mg/g per diet, Group III fructose-fed rats received ۱۰%W/V fructose, and Group IV fructose- and WS-fed rats. After eight weeks blood samples were collected to measure glucose, insulin, IL-۶, and TNF-α levels in sera. Results: Blood glucose, insulin, homeostasis model assessment for insulin resistance (HOMA-R), IL-۶, and TNF-α levels were all significantly greater in the fructose-fed rats than in the controls. Treatment with WS significantly (P < ۰.۰۵) inhibited the fructose-induced increases in glucose, insulin, HOMA-R, IL-۶, and TNF-α. Conclusion: Our data suggest that WS normalizes hyperglycemia in fructose-fed rats by reducing inflammatory markers and improving insulin sensitivity.

IL-۶, Insulin resistance, TNF- α, Withania somnifera