Mohammad Hossein Feiz Haddad - Infectious and Tropical Diseases Research Center, Health Research Institute Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Hamidreza Mahbodfar - Department of Parasitology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Zahra Zamani - Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran
Ali Ramazani - Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran

Objective(s): In an attempt to discover new natural active extracts against malaria parasites, the present study evaluated the antiplasmodial properties of selected plants based on Iranian traditional medicine. Materials and Methods: Ten plant species found in Iran were selected and collected based on the available literature about the Iranian traditional medicine. The methanolic extracts of these plants were investigated for in vitro antimalarial properties against chloroquine-sensitive (۳D۷) and multi-drug resistant (K۱) strains of Plasmodium falciparum. Their in vivo activity against Plasmodium berghei infection in mice was also determined. Cytotoxicity tests were carried out using the Raji cells line using the MTT assay. The extracts were phytochemically screened for their active constituents. Results: According to the IC۵۰ and selectivity index (SI) values, of the ۱۰ selected plant species, Citrullus colocynthis, Physalis alkekengi, and Solanum nigrum displayed potent in vitro antimalarial activity against both ۳D۷ and K۱ strains with no toxicity (IC۵۰= ۲.۰۱-۱۸.۶۷ µg/ml and SI=۳.۵۵ to ۱۹.۲۵).  Comparisons between treated and untreated control mice showed that the mentioned plant species reduced parasitemia by ۶۵.۰۸%, ۵۷.۹۷%, and ۶۰.۶۸%, respectively.  The existence of antiplasmodial compounds was detected in these plant extracts. Conclusion: This was the first study to highlight the in vitro and in vivo antiplasmodial effects of             C. colocynthis, P. alkekengi, and S. nigrum in Iran. Future studies can use these findings to design further biological tests to identify the active constituents of the mentioned plant species and clarify their mechanism of action.

Antimalarials, Cytotoxicity, Phytochemicals, Plasmodium falciparum, Traditional Medicine