Farzad Mousafarkhani - DVM Graduate, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Ali Asghar Sarchahi - Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Hadi Mohebalian - Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Javad Khoshnegah - Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Mohammad Arbabi - DVM Graduate, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

Canine distemper virus (CDV) is responsible for high morbidity and mortality in dogs worldwide. Epidemiological study of canine distemper can help to control and treat the disease in any area. This study aimed to investigate the prevalence of CDV in dogs referred to the Veterinary Hospital from September ۲۳, ۲۰۱۸ to September ۲۲, ۲۰۱۹. Dogs with at least two clinical signs of canine distemper underwent blood tests, rapid test kit from the eye and cerebrospinal fluid (CSF), and RT-PCR from whole blood and/or CSF samples. Out of ۱۲۱۲ referred dogs, ۱۱۲ dogs were suspected to have canine distemper of which ۹۰ underwent RT-PCR and rapid test kits. The disease prevalence was ۴.۰۴% (۴۹/۱۲۱۲) and ۷.۴۴% (۴۹/۶۵۹) according to the total number of referring dogs and number of referring sick dogs, respectively. The distemper fatality rate was ۶۹.۵۷% (۳۲/۴۶). Seventy percent of distemper positive cases were under ۱۲ months old and ۵۲.۰۸% were under ۶ months old. Female dogs were more susceptible than males; however, the fatality rate of males was more than females. Of distemper positive dogs, ۹۱.۸۴% were unvaccinated. The highest prevalence (۷۱.۴۳%) of dogs diagnosed with CDV occurred during the cold seasons. It is concluded that canine distemper is endemic in the geographical area of Mashhad and its prevalence rate in dogs referred to the Veterinary Hospital of Ferdowsi University of Mashhad is ۴.۰۴% and its fatality rate is ۶۹.۵۷%. This indicates that a significant number of dogs may die if they develop distemper despite treatment.

Distemper, Dog, Prevalence Rate, RT-PCR