Mahboubeh Davoudi Pahnekolayi - Horticultural Department, College of Agriculture, Ferdowsi University of Mashhad,Mashhad, Iran
Leila Samiei - Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
Ali Tehranifar - Horticultural Department, College of Agriculture, Ferdowsi University of Mashhad,Mashhad, Iran
Mahmoud Shoor - Horticultural Department, College of Agriculture, Ferdowsi University of Mashhad,Mashhad, Iran

Dog rose (Rosa canina L.) is one of the most important ornamental and medicinal plants which are usedas a rootstock for ornamental roses such as Rosa hybrid and Rosa floribunda. In vitro propagation of rosehas a very important role in rapid multiplication of species with desirable traits and in production ofhealthy and disease-free plants. Micropropagation of Rosa canina L. was revised, using its nodalsegments under different combinations of BAP (0, 0.5, 1, 1.5 and 2 mgl⁻1), GA3 (0 and 0.5 mgl⁻1) andNAA (0 and 0.5 mgl⁻1) on Murashige and Skoog (MS) and Van der Salm medium (VS) in proliferationstage and using different combinations of NAA and IBA (0, 0.3, 0.6 and 0.9 mgl⁻1) and ½ VS medium inrooting stage. The highest shoot proliferation was obtained on VS medium containing 2 mgl⁻1 BAP.Furthermore, the highest root induction obtained in ½ VS containing 0.6– 0.9 mgl⁻1 of NAA or IBA. Thepresent study presents an in vitro protocol for R. canina.

Micropropagation, Native plants, Plant growth regulators (PGRs), Rosa canina L