Abolhasan Ahmadiani - Biotechnology Research Center- Pasture Institute of Iran
Ali Haeri Rohani - Physiology department- faculty of science-Tehran University
Bahram Kazemi - Neuroscience research center-Shaheed Beheshti University -Velengac St.- Tehran
Nayere Askari - Neuroscience research center-Shaheed Beheshti University -Velengac St.- Tehran

Recent data indicated that opioid agonists in low concentration induce hyperalgesic effect. it was suggested that these paradoxical effects may be due to differences in gene expression levels of the opioid receptors. In this study we have demonstrated that the real time PCR afforded great sensitivity , accuracy and reproducibility for genes expression study in neural tissues.

Real-time PCR, opiod receptor