Majid Halvaei - Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Fatemeh Navaee - Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Amir Mohammad Ghafari - Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Payam Baei - Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.

In tissue engineering 3D bioprinters are used to pattern different cell types to construct a 3D living tissue.Different bio-inks are used to print cells. Some of them just have a supportive role in printing and after printing are removed and some of them mimic extracellular matrix (ECM) of cells. In this study, a 3D bioprinter (Royan 3D bioprinter) was designed and fabricated which support printing of three different materials including alginate that is an ionic crosslinkable material, pluronic F-127 as a sacrificial hydrogel and is thermo-responsive and also gelatin methacryloyl (GelMA) that could mimic the cell ECM and is a UV crosslinkable material. The printability of these hydrogels were tested in different concentrations and the best concentration was chosen for printing.

3D bioprinting, cell patterning, hydrogel, bio-ink, GelMA