Effect of Myo-inositol and N-acetyl-L-cysteine on processed human spermatozoa for use in modern methods of fertility treatment

Background and aims: Today, the prevalence of structural and functional disorders of sperm and subsequently the possibility of occurrenceof autism, spontaneous abortion, and infertility has increased dramatically. This study aimed to investigate the potential effects of myoinositoland N-acetyl-L-cysteine (NAC) on the improvement of processed spermatozoa indices.Methods: In this study, ۶۳ patients with male factor infertility were studied in Hazrat Zahra Infertility Center of Shahrekord. To doliquefaction, the semen sample was incubated at ۳۷°C for ۲۰ minutes. The Density Gradient Centrifugation protocol was used to processsperm. The processed sperm samples were incubated at ۳۷°C for ۲۰ minutes in four groups of control, myo-inositol (۲ mg/mL), NACantioxidant (۱۰ Mm/L) and myo-inositol+NAC. All biological parameters of sperm were evaluated using Makler slide, SQA-V spermanalyzer gold version ۲.۴۸, TUNEL assay, and Toluidine blue staining.Results: In the present study, ۶۳ men aged ۲۲-۵۸ years with infertility complaints were studied. The NAC group and the myo-inositol groupexhibited significantly higher motility in comparison with the control group (P < ۰.۰۰۱). The NAC group also had a significantly highernumber of TUNEL-negative cells and a lower number of TUNEL-positive cells than the control group (P < ۰.۰۱), but in other variables, thedifferences were not statistically significant. The morphology, S۰, S۱, S۲ and staining types in the intervention groups did not show anysignificant difference compared to those in the control group (P > ۰.۰۵).Conclusion: NAC and myo-inositol were able to improve certain indices such as sperm motility and integrity; therefore, the quality ofsperm could be improved by these drugs to treat infertility.

کلیدواژه ها:

Myo-inositol ، N-acetyl-L-cysteine ، Infertility treatment ، Biological parameters of sperm ، IVF ، ICSI